kermit-2/pCS2+
(Plasmid
#16314)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16314 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCS2+
- Backbone size w/o insert (bp) 4095
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Vector typeVertebrate expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKermit 2
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Alt nameGIPC
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Alt nameXGIPC
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SpeciesX. laevis (frog)
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Insert Size (bp)1000
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Entrez Genegipc2 (a.k.a. gipc)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For sense transcripts, cut with NotI and transcribe with SP6
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
kermit-2/pCS2+ was a gift from Peter Klein (Addgene plasmid # 16314 ; http://n2t.net/addgene:16314 ; RRID:Addgene_16314) -
For your References section:
Kermit 2/XGIPC, an IGF1 receptor interacting protein, is required for IGF signaling in Xenopus eye development. Wu J, O'Donnell M, Gitler AD, Klein PS. Development. 2006 Sep . 133(18):3651-60. 10.1242/dev.02547 PubMed 16914488