-
PurposeA plasmid with UnaG coding sequence cloned into it. UnaG gene codon optimized for E. coli and B. theta. Expresses UnaG with N-term maltose-binding protein (MBP)-tag and C-term His-tag.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163125 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepMAL-c5x
- Backbone size w/o insert (bp) 5630
- Total vector size (bp) 6088
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameUnaG
-
SpeciesAnguilla japonica
-
Insert Size (bp)438
-
GenBank ID
-
Tags
/ Fusion Proteins
- His (C terminal on insert)
- MBP (N terminal on backbone)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pMAL-c5x_UnaG was a gift from Julie Biteen (Addgene plasmid # 163125 ; http://n2t.net/addgene:163125 ; RRID:Addgene_163125) -
For your References section:
Imaging living obligate anaerobic bacteria with bilin-binding fluorescent proteins. Chia HE, Zuo T, Koropatkin NM, Marsh ENG, Biteen JS. Curr Res Microb Sci. 2020 Sep;1:1-6. doi: 10.1016/j.crmicr.2020.04.001. Epub 2020 May 11. 10.1016/j.crmicr.2020.04.001 PubMed 33313576
Map uploaded by the depositor.
