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PurposeExpresses biosensor for lactate in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 163113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1(-)
- Backbone size w/o insert (bp) 5400
- Total vector size (bp) 6690
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGreen Lindolum
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SpeciesSynthetic
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Insert Size (bp)1290
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer CMV Forward
- 3′ sequencing primer BGH Reverse (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Green Lindoblum was a gift from Takashi Tsuboi (Addgene plasmid # 163113 ; http://n2t.net/addgene:163113 ; RRID:Addgene_163113) -
For your References section:
Green fluorescent protein-based lactate and pyruvate indicators suitable for biochemical assays and live cell imaging. Harada K, Chihara T, Hayasaka Y, Mita M, Takizawa M, Ishida K, Arai M, Tsuno S, Matsumoto M, Ishihara T, Ueda H, Kitaguchi T, Tsuboi T. Sci Rep. 2020 Nov 11;10(1):19562. doi: 10.1038/s41598-020-76440-4. 10.1038/s41598-020-76440-4 PubMed 33177605
Map uploaded by the depositor.
