pRD395
(Plasmid
#163102)
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PurposeExpression of mCherry_H-NSdbd in Escherichia coli (and, potentially, other bacteria)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 163102 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBAD33
- Backbone size w/o insert (bp) 5351
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert namemCherry_H-NSdbd
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Alt namemCherry HI-NESS
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SpeciesSynthetic
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Insert Size (bp)882
- Promoter araBAD promoter
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRD395 was a gift from Remus Dame (Addgene plasmid # 163102 ; http://n2t.net/addgene:163102 ; RRID:Addgene_163102) -
For your References section:
HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein. Rashid FM, Mahlandt E, van der Vaart M, Boer DEC, Varela Alvarez M, Henneman B, Brocken DJW, Voskamp P, Blok AJ, Shimizu TS, Meijer AH, Luijsterburg MS, Goedhart J, Cremazy FGE, Dame RT. Nucleic Acids Res. 2021 Nov 4. pii: 6414584. doi: 10.1093/nar/gkab993. 10.1093/nar/gkab993 PubMed 34734265