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Addgene

pRD395
(Plasmid #163102)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163102 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBAD33
  • Backbone size w/o insert (bp) 5351
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mCherry_H-NSdbd
  • Alt name
    mCherry HI-NESS
  • Species
    Synthetic
  • Insert Size (bp)
    882
  • Promoter araBAD promoter

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRD395 was a gift from Remus Dame (Addgene plasmid # 163102 ; http://n2t.net/addgene:163102 ; RRID:Addgene_163102)
  • For your References section:

    HI-NESS: a family of genetically encoded DNA labels based on a bacterial nucleoid-associated protein. Rashid FM, Mahlandt E, van der Vaart M, Boer DEC, Varela Alvarez M, Henneman B, Brocken DJW, Voskamp P, Blok AJ, Shimizu TS, Meijer AH, Luijsterburg MS, Goedhart J, Cremazy FGE, Dame RT. Nucleic Acids Res. 2021 Nov 4. pii: 6414584. doi: 10.1093/nar/gkab993. 10.1093/nar/gkab993 PubMed 34734265