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Addgene

xfz2/pBS
(Plasmid #16310)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16310 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript II SK
  • Backbone manufacturer
    Stratagene
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    frizzled-2
  • Alt name
    fz2
  • Alt name
    xfz2
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2900
  • Entrez Gene
    fzd2 (a.k.a. frizzled2, xfz2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Isolated from head library using xfz8 as a probe. Works well for in situs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    xfz2/pBS was a gift from Peter Klein (Addgene plasmid # 16310 ; http://n2t.net/addgene:16310 ; RRID:Addgene_16310)

  • For your References section:

    Xenopus frizzled-2 is expressed highly in the developing eye, otic vesicle and somites. Deardorff MA, Klein PS. Mech Dev. 1999 Sep . 87(1-2):229-33. 10.1016/S0925-4773(99)00161-6 PubMed 10495293
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