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Addgene

kermit/pCS2+
(Plasmid #16309)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16309 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+
  • Backbone size w/o insert (bp) 4095
  • Vector type
    Vertebrate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Kermit
  • Alt name
    T2
  • Alt name
    GIPC homolog
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    1000
  • Entrez Gene
    gipc1 (a.k.a. GIPC, XGIPC, kermit)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For sense transcripts, cut with NotI and transcribe with SP6.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    kermit/pCS2+ was a gift from Peter Klein (Addgene plasmid # 16309 ; http://n2t.net/addgene:16309 ; RRID:Addgene_16309)

  • For your References section:

    Kermit, a frizzled interacting protein, regulates frizzled 3 signaling in neural crest development. Tan C, Deardorff MA, Saint-Jeannet JP, Yang J, Arzoumanian A, Klein PS. Development. 2001 Oct . 128(19):3665-74. PubMed 11585793
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