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Addgene

pEDJ-87
(Plasmid #163085)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163085 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCfB3037
  • Backbone size w/o insert (bp) 4547
  • Total vector size (bp) 7293
  • Vector type
    Yeast Expression
  • Selectable markers
    None

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VPR
  • Insert Size (bp)
    1596
  • Promoter ADH1
  • Tag / Fusion Protein
    • MCP (N terminal on insert)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer gaaattcgcttatttagaagtgtc
  • 3′ sequencing primer CTCCTTCCTTTTCGGTTAGAG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene verified sequence differs from the depositor's reference sequence. These differences do not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEDJ-87 was a gift from Michael Jensen (Addgene plasmid # 163085 ; http://n2t.net/addgene:163085 ; RRID:Addgene_163085)

  • For your References section:

    Transcriptional reprogramming in yeast using dCas9 and combinatorial gRNA strategies. Jensen ED, Ferreira R, Jakociunas T, Arsovska D, Zhang J, Ding L, Smith JD, David F, Nielsen J, Jensen MK, Keasling JD. Microb Cell Fact. 2017 Mar 15;16(1):46. doi: 10.1186/s12934-017-0664-2. 10.1186/s12934-017-0664-2 PubMed 28298224
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