Skip to main content
Addgene

pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo
(Plasmid #163084)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163084 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pPB (PiggyBac)
  • Total vector size (bp) 8991
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    anti-GFP nanobody
  • Species
    Synthetic
  • Promoter PGK

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GGCCTGGAGAAACAGCTAAA
  • 3′ sequencing primer GGAAAGGACAGTGGGAGTGG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2020.09.09.290015 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPB: PGK-H2B-mIFP-T2A-rTetR-GBP-polyA-zeo was a gift from Lacra Bintu (Addgene plasmid # 163084 ; http://n2t.net/addgene:163084 ; RRID:Addgene_163084)

  • For your References section:

    Nanobody-mediated control of gene expression and epigenetic memory. Van MV, Fujimori T, Bintu L. Nat Commun. 2021 Jan 22;12(1):537. doi: 10.1038/s41467-020-20757-1. 10.1038/s41467-020-20757-1 PubMed 33483487
Related items:
Commonly requested with: