Axin GID 1-2/pET29b
(Plasmid
#16307)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16307 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepET29b
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Backbone manufacturerEMD Biosciences
- Backbone size w/o insert (bp) 5370
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAxin GID 1-2
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Alt nameAxin
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SpeciesX. laevis (frog)
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Insert Size (bp)329
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MutationAmino acids 320-429
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Entrez Geneaxin1.L (a.k.a. XELAEV_18045581mg, axin1, fused)
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Tag
/ Fusion Protein
- His (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NcoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 Terminal (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Axin GID 1-2/pET29b was a gift from Peter Klein (Addgene plasmid # 16307 ; http://n2t.net/addgene:16307 ; RRID:Addgene_16307) -
For your References section:
Regulation of glycogen synthase kinase 3beta and downstream Wnt signaling by axin. Hedgepeth CM, Deardorff MA, Rankin K, Klein PS. Mol Cell Biol. 1999 Oct . 19(10):7147-57. PubMed 10490650