Axin DeltaRGS/pCS2MT
(Plasmid #16304)

• Depositing Lab: Peter Klein
• Publication: Hedgepeth et al Mol Cell Biol. 1999 Oct . 19(10):7147-57.

Backbone

• Vector backbone: pCS2+MT
• Backbone size w/o insert (bp): 4352
• Vector type: Vertebrate expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Axin DeltaRGS
• Alt name: Axin
• Species: X. laevis (frog)
• Insert Size (bp): 1900
• Mutation: Delta RGS: deletion of RGS domain aa 80-290
• GenBank ID: AF097313
• Entrez Gene: axin1.L (a.k.a. XELAEV_18045581mg, axin1, fused)
• Tag / Fusion Protein:
  • 5x myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dominant negative Xaxin. For sense transcripts, cut with NotI and transcribe with SP6.
To make antisense probe for whole mounts, cut with Pst1 and then remove 3' overhang before transcribing with T7.