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Addgene

pGS-21a-PcAad1
(Plasmid #163039)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163039 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGS-21a
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7300
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Depositors suggest using BL21 Star™(DE3) for protein expression and purification. 0.1 mM IPTG, 16C induction to minimize inclusion body formation
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    aryl-alcohol dehydrogenase
  • Species
    Phanerochaete chrysosporium
  • Insert Size (bp)
    1155
  • Tags / Fusion Proteins
    • GST (N terminal on insert)
    • His6 (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGS-21a-PcAad1 was a gift from Jean-Marie Francois (Addgene plasmid # 163039 ; http://n2t.net/addgene:163039 ; RRID:Addgene_163039)

  • For your References section:

    Cloning, expression and characterization of an aryl-alcohol dehydrogenase from the white-rot fungus Phanerochaete chrysosporium strain BKM-F-1767. Yang DD, Francois JM, de Billerbeck GM. BMC Microbiol. 2012 Jun 28;12:126. doi: 10.1186/1471-2180-12-126. 10.1186/1471-2180-12-126 PubMed 22742413
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