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Addgene

(517) SB KRAB-SadCas9 U6-gStop
(Plasmid #163023)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 163023 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Sleeping Beauty TET-On
  • Backbone manufacturer
    Eric Kowarz (Addgene plasmid # 60509)
  • Backbone size w/o insert (bp) 6100
  • Total vector size (bp) 9562
  • Vector type
    Mammalian Expression ; Transposon
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    34-35 degrees is ideal
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Mammalian codon-optimized SadCas9
  • Species
    S. aureus
  • Insert Size (bp)
    3483
  • Promoter Tet-On
  • Tags / Fusion Proteins
    • KRAB (N terminal on insert)
    • myc NLS (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site Not1 (not destroyed)
  • 5′ sequencing primer SB-TRE-S
  • 3′ sequencing primer SVpa-Seq-A
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Keith Joung (S. aureus dCas9 from Addgene #70703).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    (517) SB KRAB-SadCas9 U6-gStop was a gift from Tonia Rex (Addgene plasmid # 163023 ; http://n2t.net/addgene:163023 ; RRID:Addgene_163023)

  • For your References section:

    Optimization of S. aureus dCas9 and CRISPRi Elements for a Single Adeno-Associated Virus that Targets an Endogenous Gene. Backstrom JR, Sheng J, Wang MC, Bernardo-Colon A, Rex TS. Mol Ther Methods Clin Dev. 2020 Sep 6;19:139-148. doi: 10.1016/j.omtm.2020.09.001. eCollection 2020 Dec 11. 10.1016/j.omtm.2020.09.001 PubMed 33024795
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