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Addgene

Axin GID 1-2/pCS2MT
(Plasmid #16299)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16299 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone size w/o insert (bp) 4352
  • Vector type
    Vertebrate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Axin GID 1-2
  • Alt name
    Axin
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    329
  • Mutation
    GSK interaction domain aa 320-429
  • GenBank ID
    AF097313
  • Entrez Gene
    axin1.L (a.k.a. XELAEV_18045581mg, axin1, fused)
  • Tag / Fusion Protein
    • 5x myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For sense transcripts, cut with NotI and transcribe with SP6.
Potent GSK-3 inhibitor and Wnt activator.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Axin GID 1-2/pCS2MT was a gift from Peter Klein (Addgene plasmid # 16299 ; http://n2t.net/addgene:16299 ; RRID:Addgene_16299)

  • For your References section:

    Regulation of glycogen synthase kinase 3beta and downstream Wnt signaling by axin. Hedgepeth CM, Deardorff MA, Rankin K, Klein PS. Mol Cell Biol. 1999 Oct . 19(10):7147-57. PubMed 10490650
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