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Addgene

Axin/pCS2MT
(Plasmid #16298)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16298 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS2+MT
  • Backbone size w/o insert (bp) 4352
  • Vector type
    Vertebrate expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Axin
  • Alt name
    Xaxin
  • Alt name
    Flaxin
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    2529
  • GenBank ID
    AF097313
  • Entrez Gene
    axin1.L (a.k.a. XELAEV_18045581mg, axin1, fused)
  • Tag / Fusion Protein
    • 5x myc (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For sense mRNA, linearize with NotI and transcribe with SP6.
For antisense probe, cut with Pst1, remove 3' overhang, and transcribe with T7.
NcoI digest cuts out a 2333 bp fragment.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Axin/pCS2MT was a gift from Peter Klein (Addgene plasmid # 16298 ; http://n2t.net/addgene:16298 ; RRID:Addgene_16298)

  • For your References section:

    Xenopus axin interacts with glycogen synthase kinase-3 beta and is expressed in the anterior midbrain. Hedgepeth CM, Deardorff MA, Klein PS. Mech Dev. 1999 Feb . 80(2):147-51. 10.1016/S0925-4773(98)00203-2 PubMed 10072781
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