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Addgene

C453-E14: attB4-EF1p>-attB5
(Plasmid #162975)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162975 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pDonr215
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human EF1alpha promoter
  • Species
    H. sapiens (human)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    C453-E14: attB4-EF1p>-attB5 was a gift from Dominic Esposito (Addgene plasmid # 162975 ; http://n2t.net/addgene:162975 ; RRID:Addgene_162975)

  • For your References section:

    Combinatorial assembly of clone libraries using site-specific recombination. Wall VE, Garvey LA, Mehalko JL, Procter LV, Esposito D. Methods Mol Biol. 2014;1116:193-208. doi: 10.1007/978-1-62703-764-8_14. 10.1007/978-1-62703-764-8_14 PubMed 24395366
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