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Addgene

pGL2-200bp
(Plasmid #16293)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16293 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pGL2 basic
  • Backbone manufacturer
    promega
  • Backbone size w/o insert (bp) 5598
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    p53 promoter fragment
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    200
  • Mutation
    fragment -188 to +12 relative to first transcription initiation site.
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Cloning Information

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL2-200bp was a gift from Wafik El-Deiry (Addgene plasmid # 16293 ; http://n2t.net/addgene:16293 ; RRID:Addgene_16293)
  • For your References section:

    p73 or p53 directly regulates human p53 transcription to maintain cell cycle checkpoints. Wang S, El-Deiry WS. Cancer Res. 2006 Jul 15. 66(14):6982-9. 10.1158/0008-5472.CAN-06-0511 PubMed 16849542