Skip to main content
Addgene

pBS cE1 (5' dbx2 mouse cDNA) [TJ#53]
(Plasmid #16288)

Print

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16288 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pBluescript KS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5' dbx2 cDNA
  • Alt name
    dbx2
  • Alt name
    CE1
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    450
  • Mutation
    dbx2 1->415
  • Entrez Gene
    Dbx2 (a.k.a. Gm1229, AI846217, 9630005K15, 9430056A22Rik)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make an antisense probe, cut with BamHI and transcribe with T3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS cE1 (5' dbx2 mouse cDNA) [TJ#53] was a gift from Thomas Jessell (Addgene plasmid # 16288 ; http://n2t.net/addgene:16288 ; RRID:Addgene_16288)

  • For your References section:

    A sonic hedgehog-independent, retinoid-activated pathway of neurogenesis in the ventral spinal cord. Pierani A, Brenner-Morton S, Chiang C, Jessell TM. Cell. 1999 Jun 25. 97(7):903-15. 10.1016/S0092-8674(00)80802-8 PubMed 10399918
Related items:
Commonly requested with: