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Addgene

pUCP20 PilBS249*
(Plasmid #162859)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162859 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUCP20
  • Backbone size w/o insert (bp) 3643
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PilB
  • Species
    Pseudomonas aeruginosa
  • Insert Size (bp)
    1728
  • Mutation
    S249Amber
  • Tag / Fusion Protein
    • Histidine tag (H6x) (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    clone from pUCP20 PilB

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Amber mutation was inserted concomitant to replacing the original UAG stop codon with UAA from pUCP20 PilB

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUCP20 PilBS249* was a gift from Katrina Forest (Addgene plasmid # 162859 ; http://n2t.net/addgene:162859 ; RRID:Addgene_162859)
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