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Addgene

pBS chick BMP-4 [TJ#221]
(Plasmid #16285)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16285 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript KS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    BMP4
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    950
  • Entrez Gene
    BMP4 (a.k.a. BMP-4)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make an antisense probe, cut with BamHI and transcribe with T3.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS chick BMP-4 [TJ#221] was a gift from Thomas Jessell (Addgene plasmid # 16285 ; http://n2t.net/addgene:16285 ; RRID:Addgene_16285)

  • For your References section:

    Dorsal differentiation of neural plate cells induced by BMP-mediated signals from epidermal ectoderm. Liem KF, Tremml G, Roelink H, Jessell TM. Cell. 1995 Sep 22. 82(6):969-79. 10.1016/0092-8674(95)90276-7 PubMed 7553857
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