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Addgene

pFBP-4_1.sensor
(Plasmid #162844)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162844 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCS1748
  • Backbone manufacturer
    https://doi.org/10.1093/nar/gks636
  • Backbone size w/o insert (bp) 8187
  • Total vector size (bp) 8282
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    4_1_RNA-device
  • Species
    Synthetic; Tobacco ringspot virus
  • Insert Size (bp)
    108
  • GenBank ID
    4_1_RNA-device

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AvrII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer CAGGAAACAGCTATGACCATG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFBP-4_1.sensor was a gift from Matthias Heinemann (Addgene plasmid # 162844 ; http://n2t.net/addgene:162844 ; RRID:Addgene_162844)

  • For your References section:

    A synthetic RNA-based biosensor for fructose-1,6-bisphosphate that reports glycolytic flux. Ortega AD, Takhaveev V, Vedelaar SR, Long Y, Mestre-Farras N, Incarnato D, Ersoy F, Olsen LF, Mayer G, Heinemann M. Cell Chem Biol. 2021 Apr 22. pii: S2451-9456(21)00161-6. doi: 10.1016/j.chembiol.2021.04.006. 10.1016/j.chembiol.2021.04.006 PubMed 33915105
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