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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16283 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepBluescript
- Backbone size w/o insert (bp) 3000
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Vector typeMammalian Expression, Mouse Targeting
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHB9 promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)9000
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Entrez GeneMnx1 (a.k.a. HB9, Hlxb, Hlxb9, MN, MNR2)
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Tag
/ Fusion Protein
- IRES-EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHB9-MCS-IRES-EGFP (mHb9 promoter) [TJ#103] was a gift from Thomas Jessell (Addgene plasmid # 16283 ; http://n2t.net/addgene:16283 ; RRID:Addgene_16283) -
For your References section:
Conditional rhythmicity of ventral spinal interneurons defined by expression of the Hb9 homeodomain protein. Wilson JM, Hartley R, Maxwell DJ, Todd AJ, Lieberam I, Kaltschmidt JA, Yoshida Y, Jessell TM, Brownstone RM. J Neurosci. 2005 Jun 15. 25(24):5710-9. 10.1523/JNEUROSCI.0274-05.2005 PubMed 15958737
Map uploaded by the depositor.
