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pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt
(Plasmid #162821)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162821 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAS
  • Vector type
    Mammalian Expression ; PiggyBac

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ub-(N-TAG)CoV2_ORF3b_alt
  • Species
    SARS-CoV-2
  • Mutation
    CoV2_ORF3b_alt(Met1,Amber)
  • Promoter EF1alpha

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternative ORF3b as described in Hachim et. al., 2020, (doi:10.1038/s41590-020-0773-7) - Protein sequence: (TAG)AYCWRCTSCCFSERFQNHNPQKEMATSTLQGCSLCLQLAVVVCNSLLTPFARCCWP

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAS1_4x7SKPylT_EF1_Ub-*CoV2_ORF3b_alt was a gift from Simon Elsaesser (Addgene plasmid # 162821 ; http://n2t.net/addgene:162821 ; RRID:Addgene_162821)

  • For your References section:

    Universal Single-Residue Terminal Labels for Fluorescent Live Cell Imaging of Microproteins. Lafranchi L, Schlesinger D, Kimler KJ, Elsasser SJ. J Am Chem Soc. 2020 Nov 25;142(47):20080-20087. doi: 10.1021/jacs.0c09574. Epub 2020 Nov 11. 10.1021/jacs.0c09574 PubMed 33175524
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