pBS chick PEA3 [TJ#51]
(Plasmid
#16279)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 16279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBluescript
- Backbone size w/o insert (bp) 3000
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePEA3
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Alt namePea3
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SpeciesG. gallus (chicken)
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Insert Size (bp)2500
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GenBank IDAF075708
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Entrez GeneETV4 (a.k.a. PEA3)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To make an antisense probe, cut with NotI and transcribe with T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBS chick PEA3 [TJ#51] was a gift from Thomas Jessell (Addgene plasmid # 16279 ; http://n2t.net/addgene:16279 ; RRID:Addgene_16279) -
For your References section:
Functionally related motor neuron pool and muscle sensory afferent subtypes defined by coordinate ETS gene expression. Lin JH, Saito T, Anderson DJ, Lance-Jones C, Jessell TM, Arber S. Cell. 1998 Oct 30. 95(3):393-407. 10.1016/S0092-8674(00)81770-5 PubMed 9814709
Map uploaded by the depositor.