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Addgene

pET15b/GVcl 1-1066 /*TBM
(Plasmid #162781)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162781 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15b/GVcl 1-1066 (Addgene #46171)
  • Total vector size (bp) 8909
  • Modifications to backbone
    G454V
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    LB
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Gallus gallus vinculin
  • Alt name
    VCL
  • Species
    G. gallus (chicken)
  • Insert Size (bp)
    3198
  • Mutation
    changed Gly 454 to Val
  • Entrez Gene
    VCL (a.k.a. VINC1)

Cloning Information

  • Cloning method Unknown
  • 5′ sequencing primer CTGCGACGACATGGGAAAGTAGACTCTCCTGAGGCCCGT
  • 3′ sequencing primer ACGGGCCTCAGGAGAGTCTACTTTCCCATGTCGTCGCAG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    We received the plasmid bearing WT GgVcl from addgene, and then mutated it.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET15b/GVcl 1-1066 /*TBM was a gift from Laura Lafon-Hughes (Addgene plasmid # 162781 ; http://n2t.net/addgene:162781 ; RRID:Addgene_162781)

  • For your References section:

    First evidences suggesting a role of a tankyrase-binding motif (TBM) of vinculin (VCL) in epithelial cells. Vilchez-Larrea, S, Valsecchi W, Fernández-Villamil S, and Lafon Hughes L. PeerJ
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