p1.2-Hyg-Fur
(Plasmid #162774)

• Purpose: Soluble human PACE/furin protease expression
• Depositing Lab: Ivan Vorobiev
• Publication: Kovnir et al Acta Naturae. 2018 Jan-Mar;10(1):51-65.

Backbone

• Vector backbone: p1.1
• Backbone manufacturer: LMBT, LTD
• Backbone size w/o insert (bp): 11914
• Modifications to backbone: The region containing the EMCV IRES and the DHFR ORF from the p1.1 expression vector is removed. Antibiotic resistance genes and the SV40 promoter and terminator regions are added.
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human PACE/furin protease soluble
• Species: H. sapiens (human)
• Insert Size (bp): 2166
• GenBank ID: NM_002569
• Entrez Gene: FURIN (a.k.a. FUR, PACE, PCSK3, SPC1)
• Promoter: CHO EEF1A1 (Translation Elongation Factor 1 Alpha 1)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AbsI (not destroyed)
• 3′ cloning site: XbaI/NheI (destroyed during cloning)
• 5′ sequencing primer: SQ-5CH6-F GCCGCTGCTTCCTGTGAC
• 3′ sequencing primer: SQ-3CH1-R ACAAACAGTTCTGAGACCG

Resource Information

• Supplemental Documents:
  • p1.2-Hyg-Fur.gb
  • p1.2-Hyg-Fur
• A portion of this plasmid was derived from a plasmid made by: Soluble human PACE/furin ORF was obtained by PCR using plasmid SC118550 (Origene, USA) as a template, the ORF was brought in line with the reference sequence NM_002569 by adding six missing nucleotides (coding VQ).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry