Skip to main content
Addgene

p1.1-mCherry
(Plasmid #162772)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162772 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    p1.1
  • Backbone manufacturer
    LMBT, LTD
  • Backbone size w/o insert (bp) 11914
  • Modifications to backbone
    none
  • Vector type
    Mammalian Expression
  • Selectable markers
    HT/MTX

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    red fluorescent protein
  • Alt name
    mCherry
  • Species
    Synthetic; Anaplasma marginale
  • Insert Size (bp)
    804
  • Mutation
    consensus Kozak sequence (GCCGCCATGG) added before the ORF
  • GenBank ID
    KJ567138
  • Promoter CHO EEF1A1 (Translation Elongation Factor 1 Alpha 1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AbsI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer SQ-5CH6-F GCCGCTGCTTCCTGTGAC
  • 3′ sequencing primer IRESA rev aggtttccgggccctcacattg
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Insert made by PCR with pmCherry-N1 (Clontech) as a template

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p1.1-mCherry was a gift from Ivan Vorobiev (Addgene plasmid # 162772 ; http://n2t.net/addgene:162772 ; RRID:Addgene_162772)

  • For your References section:

    Approaches to Controlled Co-Amplification of Genes for Production of Biopharmaceuticals: Study of the Insertion and Amplification Dynamics of Genetic Cassettes in the Genome of Chinese Hamster Ovary Cells during Co-Expression of Compatible Pair of Plasmids. Kovnir SV, Orlova NA, Khodak Ycapital A, Cyrillic, Kondrashova MP, Gabibov AG, Skryabin KG, Vorobiev AI, Vorobiev II. Bull Exp Biol Med. 2017 Jun;163(2):245-249. doi: 10.1007/s10517-017-3776-0. Epub 2017 Jul 18. 10.1007/s10517-017-3776-0 PubMed 28726207
Related items:
Commonly requested with: