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p1.2-GS-eGFP
(Plasmid #162771)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162771 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p1.1
  • Backbone manufacturer
    LMBT, LTD
  • Backbone size w/o insert (bp) 11914
  • Total vector size (bp) 13510
  • Modifications to backbone
    The region containing the EMCV IRES and the DHFR ORF from the p1.1 expression vector is removed. The GS ORF and the SV40 promoter and terminator regions are added.
  • Vector type
    Mammalian Expression
  • Selectable markers
    L-Methionine sulfoximine (MSX)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    enhanced green fluorescent protein
  • Alt name
    eGFP
  • Species
    Aequorea victoria
  • Insert Size (bp)
    735
  • Mutation
    consensus Kozak sequence (GCCGCCATGG) added before the ORF
  • GenBank ID
    AAB02576.1
  • Promoter CHO EEF1A1 (Translation Elongation Factor 1 Alpha 1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AbsI (not destroyed)
  • 3′ cloning site NheI (not destroyed)
  • 5′ sequencing primer SQ-5CH6-F GCCGCTGCTTCCTGTGAC
  • 3′ sequencing primer SQ-3CH1-R ACAAACAGTTCTGAGACCG
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Insert made by PCR with pEGFPN2 (Clontech) as a template

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p1.2-GS-eGFP was a gift from Ivan Vorobiev (Addgene plasmid # 162771 ; http://n2t.net/addgene:162771 ; RRID:Addgene_162771)

  • For your References section:

    Approaches to Controlled Co-Amplification of Genes for Production of Biopharmaceuticals: Study of the Insertion and Amplification Dynamics of Genetic Cassettes in the Genome of Chinese Hamster Ovary Cells during Co-Expression of Compatible Pair of Plasmids. Kovnir SV, Orlova NA, Khodak Ycapital A, Cyrillic, Kondrashova MP, Gabibov AG, Skryabin KG, Vorobiev AI, Vorobiev II. Bull Exp Biol Med. 2017 Jun;163(2):245-249. doi: 10.1007/s10517-017-3776-0. Epub 2017 Jul 18. 10.1007/s10517-017-3776-0 PubMed 28726207
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