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Addgene

pGEM-sgControl_dual-MTS
(Plasmid #162765)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162765 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM-11zf(+)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    sgControl_dual-MTS
  • gRNA/shRNA sequence
    Unspecified
  • Species
    H. sapiens (human)
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer M13F
  • 3′ sequencing primer SP6
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM-sgControl_dual-MTS was a gift from Antony K Chen (Addgene plasmid # 162765 ; http://n2t.net/addgene:162765 ; RRID:Addgene_162765)

  • For your References section:

    CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci. Mao S, Ying Y, Wu X, Krueger CJ, Chen AK. Nucleic Acids Res. 2019 Nov 18;47(20):e131. doi: 10.1093/nar/gkz752. 10.1093/nar/gkz752 PubMed 31504824
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