pGEM-sgIGR_dual-MTS-3x-Set I
(Plasmid
#162764)
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PurposeSeparately expressing 3 unique sgIGR_dual-MTS (SetI)
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162764 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM-11zf(+)
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesgIGR_dual-MTS_1; sgIGR_dual-MTS_2; sgIGR_dual-MTS_3
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gRNA/shRNA sequenceUnspecified
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SpeciesH. sapiens (human)
- Promoter U6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer M13F
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM-sgIGR_dual-MTS-3x-Set I was a gift from Antony K Chen (Addgene plasmid # 162764 ; http://n2t.net/addgene:162764 ; RRID:Addgene_162764) -
For your References section:
CRISPR/dual-FRET molecular beacon for sensitive live-cell imaging of non-repetitive genomic loci. Mao S, Ying Y, Wu X, Krueger CJ, Chen AK. Nucleic Acids Res. 2019 Nov 18;47(20):e131. doi: 10.1093/nar/gkz752. 10.1093/nar/gkz752 PubMed 31504824
