SL2-sgSat-MTSb/BFP/pdCas9-C1
(Plasmid
#162761)
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PurposeExpressing dCas9 and sgRNA containg MTSa targeting centromeres
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162761 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedCas9 and sgRNA(SL2-sgSat-MTSb)
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gRNA/shRNA sequenceGAATCTGCAAGTGGATATTGTTTGAGAGCTATGCTGGAAACAGCATAGCAAGTTCA AATAAGGCTAGTCCGTTATCAACTTGGCCCCGGAGCAGAAGACGTCACGACATCA CTTACGCTGAGTAGCTGCAGTCTGCTCCGGGGCCAGTGGCACCGAGTCGGTGCTT TTTTT
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SpeciesH. sapiens (human)
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MutationdCas9(nuclease deactivated Cas9)
- Promoter U6/CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer LKO 1-5 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SL2-sgSat-MTSb/BFP/pdCas9-C1 was a gift from Antony K Chen (Addgene plasmid # 162761 ; http://n2t.net/addgene:162761 ; RRID:Addgene_162761) -
For your References section:
A CRISPR/molecular beacon hybrid system for live-cell genomic imaging. Wu X, Mao S, Yang Y, Rushdi MN, Krueger CJ, Chen AK. Nucleic Acids Res. 2018 Jul 27;46(13):e80. doi: 10.1093/nar/gky304. 10.1093/nar/gky304 PubMed 29718399
