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Addgene

SL2-sgTelo-MTSa/BFP/pdCas9-C1
(Plasmid #162760)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162760 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pEGFP-C1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    dCas9 and sgRNA(SL2-sgTelo-MTSa)
  • gRNA/shRNA sequence
    GTTAGGGTTAGGGTTAGGGTTAGTTTGAGAGCTATGCTGGAAACAGCATAGCAAGT TCAAATAAGGCTAGTCCGTTATCAACTTGGCCCCGGAGCAGAACGACAGGAGTTG TGTTTGTGGACGAAGAGCCTGCAGTCTGCTCCGGGGCCAGTGGCACCGAGTCGG TGCTTTTTTT
  • Species
    H. sapiens (human)
  • Mutation
    dCas9(nuclease deactivated Cas9)
  • Promoter U6/CMV

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SL2-sgTelo-MTSa/BFP/pdCas9-C1 was a gift from Antony K Chen (Addgene plasmid # 162760 ; http://n2t.net/addgene:162760 ; RRID:Addgene_162760)
  • For your References section:

    A CRISPR/molecular beacon hybrid system for live-cell genomic imaging. Wu X, Mao S, Yang Y, Rushdi MN, Krueger CJ, Chen AK. Nucleic Acids Res. 2018 Jul 27;46(13):e80. doi: 10.1093/nar/gky304. 10.1093/nar/gky304 PubMed 29718399