pMSCVII-LO
(Plasmid
#162754)
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PurposeRetroviral (MSCV) expression of LSSmOrange fluorescent protein
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162754 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMSCVII
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Backbone manufacturerMaki Nakayama
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLSSmOrange
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SpeciesOther
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer 5pMIG
- 3′ sequencing primer 3LTR-2 (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMSCVII-LO was a gift from Maki Nakayama (Addgene plasmid # 162754 ; http://n2t.net/addgene:162754 ; RRID:Addgene_162754) -
For your References section:
Multiplex T-cell Stimulation Assay Utilizing a T-cell Activation Reporter-based Detection System. Landry LG, Mann SE, Anderson AM, Nakayama M. Bio Protoc. 2021 Jan 20;11(2):e3883. doi: 10.21769/BioProtoc.3883. eCollection 2021 Jan 20. 10.21769/BioProtoc.3883 PubMed 33732772