8xNFAT-ZsG-muhCD4
(Plasmid
#162745)
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PurposeNFAT-driven ZsGreen-1 reporter gene with mutated human CD4 gene
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 162745 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSIR
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Backbone manufacturerMaki Nakayama
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Vector typeMammalian Expression, Retroviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene
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SpeciesH. sapiens (human)
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MutationHuman CD4 gene with glutamine to tyrosine at position 40 and threonine to tryptophan at position 45
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Entrez GeneCD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Second gene is mutated human CD4
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
8xNFAT-ZsG-muhCD4 was a gift from Maki Nakayama (Addgene plasmid # 162745 ; http://n2t.net/addgene:162745 ; RRID:Addgene_162745) -
For your References section:
Proinsulin-Reactive CD4 T Cells in the Islets of Type 1 Diabetes Organ Donors. Landry LG, Anderson AM, Russ HA, Yu L, Kent SC, Atkinson MA, Mathews CE, Michels AW, Nakayama M. Front Endocrinol (Lausanne). 2021 Mar 25;12:622647. doi: 10.3389/fendo.2021.622647. eCollection 2021. 10.3389/fendo.2021.622647 PubMed 33841327