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Addgene

8xNFAT-ZsG-muhCD4
(Plasmid #162745)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162745 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSIR
  • Backbone manufacturer
    Maki Nakayama
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene
  • Species
    H. sapiens (human)
  • Mutation
    Human CD4 gene with glutamine to tyrosine at position 40 and threonine to tryptophan at position 45
  • Entrez Gene
    CD4 (a.k.a. CD4mut, IMD79, Leu-3, OKT4D, T4)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Second gene is mutated human CD4

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    8xNFAT-ZsG-muhCD4 was a gift from Maki Nakayama (Addgene plasmid # 162745 ; http://n2t.net/addgene:162745 ; RRID:Addgene_162745)

  • For your References section:

    Proinsulin-Reactive CD4 T Cells in the Islets of Type 1 Diabetes Organ Donors. Landry LG, Anderson AM, Russ HA, Yu L, Kent SC, Atkinson MA, Mathews CE, Michels AW, Nakayama M. Front Endocrinol (Lausanne). 2021 Mar 25;12:622647. doi: 10.3389/fendo.2021.622647. eCollection 2021. 10.3389/fendo.2021.622647 PubMed 33841327
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