pBa.Halo-Kif13B tail 442-1843
(Plasmid #162721)

• Purpose: Mammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residues
• Depositing Lab: Marvin Bentley
• Publication: Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.

Backbone

• Vector backbone: pBeta Actin-Halo
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Kif 13B
• Species: M. musculus (mouse)
• Mutation: WT with deletion of the 441 N-terminal residues.
• GenBank ID: NM_001081177.1
• Entrez Gene: Kif13b (a.k.a. 5330429L19Rik, 6030414C01, C130021D12Rik, GAKIN)
• Promoter: Chicken Beta actin

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.