pBS mouse GDF7 [TJ#37]
(Plasmid
#16272)
-
Depositing Lab
-
Publication
-
Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 16272 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepBluescript KS
- Backbone size w/o insert (bp) 3000
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameGDF7
-
SpeciesM. musculus (mouse)
-
Insert Size (bp)519
-
Entrez GeneGdf7 (a.k.a. BMP1, BMP12)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer T3
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To make a sense probe, cut with XbaI and transcribe with T3. To make an antisense probe, cut with EcoRI and transcribe with T7.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pBS mouse GDF7 [TJ#37] was a gift from Thomas Jessell (Addgene plasmid # 16272 ; http://n2t.net/addgene:16272 ; RRID:Addgene_16272)
Map uploaded by the depositor.