pBa. BDNF-Halo
(Plasmid
#162718)
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PurposeMammalian expression of rat BDNF
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 162718 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBeta Actin-Halo
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameBrain derived neurotrophic factor
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SpeciesR. norvegicus (rat)
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Mutationnone
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GenBank IDNM_001270630.1
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Entrez GeneBdnf
- Promoter Chicken Beta actin
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Tag
/ Fusion Protein
- Halo (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBa. BDNF-Halo was a gift from Marvin Bentley (Addgene plasmid # 162718 ; http://n2t.net/addgene:162718 ; RRID:Addgene_162718) -
For your References section:
A Novel Labeling Strategy Reveals That Myosin Va and Myosin Vb Bind the Same Dendritically Polarized Vesicle Population. Frank M, Citarella CG, Quinones GB, Bentley M. Traffic. 2020 Sep 21. doi: 10.1111/tra.12764. 10.1111/tra.12764 PubMed 32959500