pBa.Halo-LDLR
(Plasmid #162717)

• Purpose: Mammalian expression of human LDLR
• Depositing Lab: Marvin Bentley
• Publication: Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.

Backbone

• Vector backbone: pBeta Actin-Halo
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: LDLR
• Species: H. sapiens (human)
• Mutation: none
• GenBank ID: BC014514.1
• Entrez Gene: LDLR (a.k.a. FH, FHC, FHCL1, LDLCQ2)
• Promoter: Chicken Beta actin
• Tag / Fusion Protein:
  • Halo (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.