pBa.Halo-myosin Va tail 1006-1828
(Plasmid #162715)

• Purpose: Mammalian expression of rat WT Myosin Va with deletion of the 1005 N-terminal residues
• Depositing Lab: Marvin Bentley
• Publication: Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.

Backbone

• Vector backbone: pBeta Actin-Halo
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myosin Va
• Species: R. norvegicus (rat)
• Mutation: WT with deletion of the 1005 N-terminal residues.
• GenBank ID: NP_071514.2
• Entrez Gene: Myo5a (a.k.a. Dop, Myh12)
• Promoter: Chicken Beta actin
• Tag / Fusion Protein:
  • Halo (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBa vector is susceptible to recombination and should not be grown in fast growing bacteria or cloned using recombination.