pBa.GFP-myosin Vb tail 1436-1846
(Plasmid #162714)

• Purpose: Mammalian expression of rat WT Myosin Vb with deletion of the 1435 N-terminal residues
• Depositing Lab: Marvin Bentley
• Publication: Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.12764.

Backbone

• Vector backbone: pBeta Actin-GFP
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Myosin Vb
• Species: R. norvegicus (rat)
• Mutation: WT with deletion of the 1435 N-terminal residues.
• GenBank ID: NM_017083.1
• Entrez Gene: Myo5b (a.k.a. Myr6)
• Promoter: Chicken Beta actin
• Tag / Fusion Protein:
  • GFP (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pBa vector is AV4 to recombination and should not be grown in fast growing bacteria or cloned using recombination.