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Addgene

pBS chick LH-2B [TJ#34]
(Plasmid #16271)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16271 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pBluescript KS
  • Backbone size w/o insert (bp) 3000
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LH2B
  • Alt name
    LH-2B
  • Alt name
    Lhx9
  • Species
    G. gallus (chicken)
  • GenBank ID
    L35566
  • Entrez Gene
    LHX9 (a.k.a. LH-2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T3
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

full length LH-2B.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS chick LH-2B [TJ#34] was a gift from Thomas Jessell (Addgene plasmid # 16271 ; http://n2t.net/addgene:16271 ; RRID:Addgene_16271)
  • For your References section:

    A role for the roof plate and its resident TGFbeta-related proteins in neuronal patterning in the dorsal spinal cord. Liem KF, Tremml G, Jessell TM. Cell. 1997 Oct 3. 91(1):127-38. 10.1016/S0092-8674(01)80015-5 PubMed 9335341