pGEM mouse dbx-1 [TJ#11]
(Plasmid
#16269)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16269 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEM
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 2700
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namedbx1 3'UTR
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Alt namedbx-1
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SpeciesM. musculus (mouse)
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Insert Size (bp)850
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Entrez GeneDbx1 (a.k.a. Dbx, AI426026)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
To make an antisense probe, cut with EcoRI and transcribe with T7.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEM mouse dbx-1 [TJ#11] was a gift from Thomas Jessell (Addgene plasmid # 16269 ; http://n2t.net/addgene:16269 ; RRID:Addgene_16269) -
For your References section:
A sonic hedgehog-independent, retinoid-activated pathway of neurogenesis in the ventral spinal cord. Pierani A, Brenner-Morton S, Chiang C, Jessell TM. Cell. 1999 Jun 25. 97(7):903-15. 10.1016/S0092-8674(00)80802-8 PubMed 10399918
Map uploaded by the depositor.
