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Addgene

pGEM rat islet1 (phoII) [TJ#1]
(Plasmid #16268)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16268 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEM3
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 2700
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    islet1
  • Alt name
    isl1
  • Alt name
    islet-1
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    1500
  • Entrez Gene
    Isl1 (a.k.a. Isl-1, isl-1=homeobox)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer SP6
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

To make a sense probe, cut with XbaI and transcribe with SP6. To make an antisense probe, cut with HindIII and transcribe with T7.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGEM rat islet1 (phoII) [TJ#1] was a gift from Thomas Jessell (Addgene plasmid # 16268 ; http://n2t.net/addgene:16268 ; RRID:Addgene_16268)
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