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pSUPER.retro-{beta}4 siRNA
(Plasmid #16266)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16266 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSUPER.retro
  • Backbone manufacturer
    Oligoengine
  • Backbone size w/o insert (bp) 7641
  • Vector type
    Mammalian Expression, Retroviral, RNAi
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    SIRNA Beta 4 Integrin
  • Alt name
    beta 4 integrin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    60
  • Entrez Gene
    ITGB4 (a.k.a. CD104, GP150, JEB5A, JEB5B)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer 5'-GGAAGCCTTGGCTTTTG-3'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The following oligonucleotides were annealed and ligated into pSUPER between the BglII and HindIII sites: beta4 siRNA, 5'-gatcccc GAGCTGCACGGAGTGTGTC ttcaagaga GACACACTCCGTGCAGCTC tttttggaaa-3' and 5'-agcttttccaaaaa GAGCTGCACGGAGTGTGTC tctcttgaa GACACACTCCGTGCAGCTC ggg-3'.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSUPER.retro-{beta}4 siRNA was a gift from Arthur Mercurio (Addgene plasmid # 16266 ; http://n2t.net/addgene:16266 ; RRID:Addgene_16266)

  • For your References section:

    The Met receptor and alpha 6 beta 4 integrin can function independently to promote carcinoma invasion. Chung J, Yoon SO, Lipscomb EA, Mercurio AM. J Biol Chem. 2004 Jul 30. 279(31):32287-93. 10.1074/jbc.M403809200 PubMed 15161909
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