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Addgene

pRc/CMV-Beta(4)Integrin
(Plasmid #16265)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 16265 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRc/CMV expression vector
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Beta 4 Integrin
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    5700
  • Mutation
    lacked the 70- and 7-amino acid splice variants and the 5` upstream 49-base pair region
  • Entrez Gene
    ITGB4 (a.k.a. CD104, GP150, JEB5A, JEB5B)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRc/CMV-Beta(4)Integrin was a gift from Arthur Mercurio (Addgene plasmid # 16265 ; http://n2t.net/addgene:16265 ; RRID:Addgene_16265)

  • For your References section:

    Activation of the p21 pathway of growth arrest and apoptosis by the beta 4 integrin cytoplasmic domain. Clarke AS, Lotz MM, Chao C, Mercurio AM. J Biol Chem. 1995 Sep 29. 270(39):22673-6. 10.1074/jbc.270.39.22957 PubMed 7559386
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