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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 16263 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepED
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIRE1
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SpeciesH. sapiens (human)
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Mutationisoleucine to glycine mutation at residue 642
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Entrez GeneERN1 (a.k.a. IRE1, IRE1P, IRE1a, hIRE1p)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRandy Kaufman lab created original pED-hIRE1 vector (see Tirasophon et al., Genes Dev 1998).
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
I642G mutation of pED-hIRE1 plasmid
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pED-hIRE1(I642G) was a gift from Peter Walter (Addgene plasmid # 16263 ; http://n2t.net/addgene:16263 ; RRID:Addgene_16263) -
For your References section:
IRE1 signaling affects cell fate during the unfolded protein response. Lin JH, Li H, Yasumura D, Cohen HR, Zhang C, Panning B, Shokat KM, Lavail MM, Walter P. Science. 2007 Nov 9. 318(5852):944-9. 10.1126/science.1146361 PubMed 17991856
