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Addgene

pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V
(Plasmid #162618)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162618 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pC2+
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TARDBP
  • Species
    H. sapiens (human)
  • Mutation
    M337V
  • Entrez Gene
    TARDBP (a.k.a. ALS10, TDP-43)

Cloning Information

  • Cloning method Unknown

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

mCerulean156-239 fused to TARDBP

Please visit https://www.biorxiv.org/content/10.1101/2020.10.08.330894v1 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2plus-mCerulean 156-239-GGGS-TDP-43 M337V was a gift from Marco Morsch (Addgene plasmid # 162618 ; http://n2t.net/addgene:162618 ; RRID:Addgene_162618)
  • For your References section:

    In vivo Validation of Bimolecular Fluorescence Complementation (BiFC) to Investigate Aggregate Formation in Amyotrophic Lateral Sclerosis (ALS). Don EK, Maschirow A, Radford RAW, Scherer NM, Vidal-Itriago A, Hogan A, Maurel C, Formella I, Stoddart JJ, Hall TE, Lee A, Shi B, Cole NJ, Laird AS, Badrock AP, Chung RS, Morsch M. Mol Neurobiol. 2021 May;58(5):2061-2074. doi: 10.1007/s12035-020-02238-0. Epub 2021 Jan 7. 10.1007/s12035-020-02238-0 PubMed 33415684