pCMV-EPN-01(HiBiT)
(Plasmid
#162590)
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PurposeEPN-01 tagged with HiBiT tag under CMV promoter
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 162590 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEPN-01
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SpeciesSynthetic
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Insert Size (bp)852
- Promoter CMV promoter
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Tag
/ Fusion Protein
- HiBiT
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-EPN-01(HiBiT) was a gift from Masaharu Somiya (Addgene plasmid # 162590 ; http://n2t.net/addgene:162590 ; RRID:Addgene_162590) -
For your References section:
Real-Time Luminescence Assay for Cytoplasmic Cargo Delivery of Extracellular Vesicles. Somiya M, Kuroda S. Anal Chem. 2021 Mar 24. doi: 10.1021/acs.analchem.1c00339. 10.1021/acs.analchem.1c00339 PubMed 33759512
