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Addgene

pcDNA3.1-LgBiT
(Plasmid #162588)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162588 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Large subunit of NanoLuc (LgBiT)
  • Species
    Synthetic
  • Insert Size (bp)
    480
  • Promoter CMV promoter

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-LgBiT was a gift from Masaharu Somiya (Addgene plasmid # 162588)

  • For your References section:

    Real-Time Luminescence Assay for Cytoplasmic Cargo Delivery of Extracellular Vesicles. Somiya M, Kuroda S. Anal Chem. 2021 Mar 24. doi: 10.1021/acs.analchem.1c00339. 10.1021/acs.analchem.1c00339 PubMed 33759512
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