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pFastBac1_GI.1_N167C-L169C
(Plasmid #162587)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162587 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pFastBac1
  • Backbone manufacturer
    ThermoFisher
  • Backbone size w/o insert (bp) 4776
  • Total vector size (bp) 6379
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    VP1 protein from human norovirus GI.1
  • Alt name
    58 kd capsid protein [Norwalk virus]
  • Species
    Norovirus
  • Insert Size (bp)
    1590
  • Mutation
    Asparagine 167 changed to Cysteine and Leucine 169 changed to Cysteine
  • GenBank ID
  • Promoter polyhedrin

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pFastBac1_GI.1_N167C-L169C was a gift from Peter Kwong (Addgene plasmid # 162587 ; http://n2t.net/addgene:162587 ; RRID:Addgene_162587)

  • For your References section:

    Disulfide stabilization of human norovirus GI.1 virus-like particles focuses immune response toward blockade epitopes. Verardi R, Lindesmith LC, Tsybovsky Y, Gorman J, Chuang GY, Edwards CE, Brewer-Jensen PD, Mallory ML, Ou L, Schon A, Shi W, Tully ES, Georgiou G, Baric RS, Kwong PD. NPJ Vaccines. 2020 Dec 14;5(1):110. doi: 10.1038/s41541-020-00260-w. 10.1038/s41541-020-00260-w PubMed 33318483
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