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pARC8-LambdaRedBeta-EcSSB-EcMutLE32K
(Plasmid #162576)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 162576 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pARC8
  • Backbone size w/o insert (bp) 3298
  • Total vector size (bp) 6507
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Lambda Red-Beta
  • Alt name
    RedBeta
  • Alt name
    Beta
  • Species
    E. coli
  • Insert Size (bp)
    786
  • GenBank ID
    WP_000100844
  • Promoter pBAD

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACA GCG TCA CAC TTT GCA AAG
  • 3′ sequencing primer CCT GAA GTC AGC CCC ATA C
    • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    E. coli SSB
  • Alt name
    SSB
  • Species
    E. coli
  • Insert Size (bp)
    537
  • GenBank ID
    AP009048
  • Promoter pBAD

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACA GCG TCA CAC TTT GCA AAG
  • 3′ sequencing primer CCT GAA GTC AGC CCC ATA C
    • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    EcMutLE32K
  • Alt name
    MutL
  • Species
    E. coli
  • Insert Size (bp)
    1848
  • Mutation
    E32K mutated to give dominant negative phenotype
  • GenBank ID
    AP009048
  • Promoter pBAD

Cloning Information for Gene/Insert 3

  • Cloning method Gibson Cloning
  • 5′ sequencing primer ACA GCG TCA CAC TTT GCA AAG
  • 3′ sequencing primer CCT GAA GTC AGC CCC ATA C
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pARC8-LambdaRedBeta-EcSSB-EcMutLE32K was a gift from George Church (Addgene plasmid # 162576 ; http://n2t.net/addgene:162576 ; RRID:Addgene_162576)

  • For your References section:

    Characterizing the portability of phage-encoded homologous recombination proteins. Filsinger GT, Wannier TM, Pedersen FB, Lutz ID, Zhang J, Stork DA, Debnath A, Gozzi K, Kuchwara H, Volf V, Wang S, Rios X, Gregg CJ, Lajoie MJ, Shipman SL, Aach J, Laub MT, Church GM. Nat Chem Biol. 2021 Jan 18. pii: 10.1038/s41589-020-00710-5. doi: 10.1038/s41589-020-00710-5. 10.1038/s41589-020-00710-5 PubMed 33462496
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